PREPARATION OF SOLUBLE CHOLINE DEHYDROGENASE FROM LIVER MITOCHONDRIA
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منابع مشابه
Preparation and partial purification of soluble choline dehydrogenase from liver mitochondria.
Choline dehydrogenase is the initial enzyme of the complete choline oxidase system. The latter includes all agents and enzymes involved in hydrogen transport to oxygen plus the initial dehydrogenase. Problems concerning the properties of choline dehydrogenase have remained unsolved largely because of the insoluble nature of the enzyme. It has resisted solution by such techniques as freezing and...
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Mouse liver mitochondria were prepared as described and resuspended in MRM buffer (250 mM sucrose, 10 mM HEPES, pH 7.5, 1 mM ATP, 5 mM sodium succinate, 80 mM ADP, 2 mM K2HPO4) at a concentration of 0.5 mg ml 21 (ref. 18). Rat liver mitochondria were isolated from 4–6-month-old Fischer 344 £ brown Norway F1 rats by differential centrifugation as described, except the final wash and resuspension...
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Aldehyde dehydrogenase from sheep liver mitochondria was purified to homogeneity as judged by electrophoresis on polyacrylamide gels, and by sedimentation-equilibrium experiments in the analytical ultracentrifuge. The enzyme has a molecular weight of 198000 and a subunit size of 48000, indicating that the molecule is a tetramer. Fluorescence and spectrophotometric titrations indicate that each ...
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Kinetic studies of alcohol dehydrogenase prepared from horse liver by different methods have given different results (Theorell, Nygaard & Bonnichsen, 1955; Dalziel, 1958; Theorell, 1958; K. Dalziel, unpublished work, 1957). The most active crystalline preparations have been obtained by a modification of earlier methods (Bonnichsen, 1950; Bonnichsen & Brink, 1955) in which the principal new feat...
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Dimethylglycine dehydrogenase (EC 1.5.99.2) and sarcosine dehydrogenase (EC 1.5.99.1) are the folate binding proteins of rat liver mitochondria. These two enzymes contain covalently bound flavin and catalyze similar oxidative demethylation reactions (Wittwer, A. J., and Wagner, C. (1981) J. Biol. Chem. 256, 4102-4108). Flavin-peptides have been purified from these two enzymes after proteolytic ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1953
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)52359-6